| CYR61

Cysteine-rich protein 61, also known as Cyr61 or CCN family member 1 (CCN1), is a matricellular protein encoded by the Cyr61 gene. It belongs to the CCN family of secreted extracellular matrix (ECM)-associated signaling proteins (CCN intercellular signaling proteins). By interacting with cell surface integrin receptors and heparan sulfate proteoglycans, it regulates a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence. During embryonic development, it is essential for cardiac septal morphogenesis, vascular formation, and vascular integrity in the placenta. In adulthood, it plays a significant role in inflammation and tissue repair, and has been implicated in chronic inflammation-related diseases, including rheumatoid arthritis, atherosclerosis, diabetic nephropathy and retinopathy, as well as various forms of cancer.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	400	200	100	50	25	12.5	6.25	0
OD	2.36	1.78	1.23	0.79	0.46	0.29	0.17	0.09
Corrected OD	2.27	1.69	1.14	0.7	0.37	0.2	0.08	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse CYR61 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Tissue Homogenates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse CYR61 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-95	85-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	101	108